a Record control number 000307981500002

a ISSN 1572-3887

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QU4.3

b Item number .E93 2012

a Personal name Dalmızrak, Özlem,

h Medium Electronic Document

a Title Evaluation of the in vitro Inhibitory Impact of Hypericin on Placental Glutathione S-Transferase pi.

c Statement of responsibility, etc Ozlem Dalmizrak, Gulnihal Kulaksiz, Erkmen Nazmi Ozer.

c Date of publication, distribution, etc 2012.

b Name of publisher, distributor, etc Springer,

a Place of publication, distribution, etc New York :

a Summary, etc St John's Wort (SJW) extracts are herbal products which are available without prescription in most countries and widely used in the treatment of mild to moderate depression. Since it is a herbal product and available without prescription, use of SJW is common among pregnant and/or lactating woman. The principal of the study was to clarify the effects of hypericin, one of the components of SJW, on glutathione S-transferase-pi (GST-pi) purified from human placenta. The K (m) values of GST-pi were 0.21 +/- A 0.03 mM for glutathione (GSH) and 2.29 +/- A 0.54 mM for 1-chloro-2,4-dinitrobenzene (CDNB). At fixed [GSH], the V (m) value calculated was about 3 times higher than the conditions in which [CDNB] was fixed; 201 +/- A 30 U/mg protein versus 74 +/- A 3 U/mg protein. At constant substrate concentrations (1 mM), an average IC (50) value of 0.70 +/- A 0.02 mu M was obtained. Hypericin inhibited GST-pi competitively with respect to both substrates. When GSH was the varied substrate a K (i) value of 0.31 +/- A 0.05 mu M was found; when CDNB was the varied substrate, a K (i) value of 0.85 +/- A 0.02 mu M was obtained. On the basis of these data considering transplacental transfer of hypericin and immature hepatic clearance of the baby, using this herbal product may cause abnormalites due to the inhibition of one of the most important placental detoxification enzymes, GST-pi.

a Topical term or geographic name as entry element Biochemistry

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Inhibition kinetics

a Topical term or geographic name as entry element St John's Wort

a Topical term or geographic name as entry element Transplacental transfer

a Main entry heading Protein Journal

g Relationship information OCT 2012, Vol 31 Issue 7, p544-549

x International Standard Serial Number 15723887

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1007/s10930-012-9433-6